| マテリアル番号 | IUM2013-0002 |
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| マテリアル分類 |
遺伝子・タンパク質(微生物)
...
ヒト由来を含まない
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| マテリアル名 | ALSV (Apple latent spherical virus) |
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| リンゴ小球形潜在ウイルス |
| マテリアル関連情報 |
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| 機能 | This ALSV vector is a tool which can expression exogenous genes simply with a vegetable object using infection and reproductive activity of a virus.
It is not necessary to carry out a tissue culture.
It can use widely in applicable fields, such as basic research, such as gene function analysis and medical production.
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| ウイルスの感染・増殖能を利用して外来遺伝子を植物体で簡便に発現できるツールです。
組織培養を行う必要は無く、遺伝子の機能解析などの基礎研究から医薬品の生産などの
応用分野で広く利用可能です。
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| REFERENCES | Vector Sequence: Apple latent spherical virus RNA1 from Apple latent spherical virus (ALSV) – ALSV RNA1 includes a single open reading frame encoding protease-cofactor, helicase, cysteine protease, and RNA polymerase (Igarashi et al 2009, Virology 386:407-416) |
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| 参考文献ID |
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| 詳細表示エリア |
| 備考 | The ALSV vector (RNA2) has been engineered to create a cloning site for introduction of gene fragments to induce gene silencing.
The Apple latent spherical virus (ALSV) gene silencing vector will be used to test gene function in cucumber and melon plants. Sequence fragments of genes to be silenced will be inserted into the vector cloning site in pEALSR2LR5 in the ALSV RNA2 as described on Igarashi et al. 2009 (Virology 286:407-416).
The ALSV VIGS vectors (RNA1 and RNA2) will be amplified in E. coli, plasmids purified, and mechanically inoculated onto leaves of Chenopdium quinoa in the growth chamber. To test for gene silencing infected C. quinoa leaves will be used to mechanically inoculate cucumber or melon in the growth chamber or greenhouse.
ALSV is a useful silencing vector because it does not cause symptoms or disease, including on apple (Li et al., 2004, Archives Virology 149:1541-1558).
Although ALSV can experimentally infect a relatively wide range of plant species, natural infection of ALSV has only been documented in one apple tree in Morioka, Japan and did not spread in the orchard over a period of more than two decades (Nakamura et al., 2011., J Gen Plant Pathol: 7748-53).
The virus did not have any obvious symptoms in apple trees inoculate by grafting. No other natural hosts have been identified.
The ALSV vector (RNA2) has been engineered to create a cloning site for introduction of gene fragments to induce gene silencing.
The Apple latent spherical virus (ALSV) gene silencing vector will be used to test gene function in cucumber and melon plants. Sequence fragments of genes to be silenced will be inserted into the vector cloning site in pEALSR2LR5 in the ALSV RNA2 as described on Igarashi et al. 2009 (Virology 286:407-416).
The ALSV VIGS vectors (RNA1 and RNA2) will be amplified in E. coli, plasmids purified, and mechanically inoculated onto leaves of Chenopdium quinoa in the growth chamber. To test for gene silencing infected C. quinoa leaves will be used to mechanically inoculate cucumber or melon in the growth chamber or greenhouse.
ALSV is a useful silencing vector because it does not cause symptoms or disease, including on apple (Li et al., 2004, Archives Virology 149:1541-1558).
Although ALSV can experimentally infect a relatively wide range of plant species, natural infection of ALSV has only been documented in one apple tree in Morioka, Japan and did not spread in the orchard over a period of more than two decades (Nakamura et al., 2011., J Gen Plant Pathol: 7748-53).
The virus did not have any obvious symptoms in apple trees inoculate by grafting. No other natural hosts have been identified.
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| 知的財産権の有無 |
[あり]
| 'あり'の場合 |
出願状況 | 出願準備中 |
| 権利の種類 | --- 未選択 --- |
| 出願番号、公開番号等 | |
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