a human hepatoma cell line with heat-inducible enhanced liver functions by introducing eight human-derived liver-enriched transcription factor genes (HepG2-based)
donor plasmid for gene integlation using Cre recombinase
donor plasmid for gene integlation using Cre recombinase
Hepatoma cell (Hepa1-6) induced 8 sorts of transcription factors' genes which secveral liver functionsare up-ragulated by an addintion of DOXY.
We generated transgenic Chlamydomonas strain with retargetable genomic transgene integration using Cre-loxP system. The established cells can be expected to be used not only for basic research such as photosynthesis but also as a production host for the high and stable production of recombinant proteins.
recombinat E. coli alkaliphosphatase (AP) containing a bridge-building site (Y-tag) by a reaction with trans-glutaminase in the C terminus of AP
recombinat E. coli alkaliphosphatase (AP) containing a bridge-building site (NK-tag) by a reaction with trans-glutaminase in the N terminus of AP
Recombinant protein G and protein A with Y-tag that can be recognized by peroxidase-catalyzed tyrosine coupling reaction
Recombinant streptavidin with Y-tag that can be recognized by peroxidase-catalyzed coupling reaction

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