ligD, argB, and Sc triple disruptant of Aspergillus kawachii IFO 4308, a host microorganism for efficient gene targeting

nkuB disruptant of Aspergillus nidulans A89, a host microorganism for efficient gene targeting

wscA and wscB double disruptant of Aspergillus nidulans AKU89, cell wall mutant strain

Integrative shuttle vector in E. coli-Geobacillus. For gene insertion into Geobacillus kaustophilus HTA426 chromosome. This plasmid is consisted with pUC replicon, ampicilin resistance gene, uracil auxotrophic gene marker, gk704 promoter and GK0707 insertion region.

recombinant plasmid containing oriT into pUCG18 (Taylor, M. P., C. D. Esteban, and D. J. Leak. 2008, Development of a versatile shuttle vector for gene expression in Geobacillus spp. Plasmid 60: 45-52.), E. coli-Geobacillus shuttle vector. The plasmid consists of pUC replicon, ampicilin resistant gene, thermostable kanamycin nucleotidyltransferase gene (TK101), pBST1 replicon and oriT.

E.coli-Geobacillus shuttle plasmid derivered from pUC19. The plasmid consists of pUC replicon, thermostable kanamycin nucleotidyltransferase gene (TK101) and oriT.

Geobacillus kaustophilus HTA426 derivative lacking uracil biosynthesis genes (pyrF,pyrR) and restriction-modification system genes（GK1378-1390, GK0343-0346).

This strain is resulted from transformation with IncP1 plasmid pUB307 (Bennett, P. M., J. Grinsted, and M. H. Richmond. 1977. Transposition of TnA does not generate deletions. Mol. Gen. Genet. 154: 205-211) to Escherichia coli IR27, derivative of E. coli strain K, carrying DNA methylase genes (GK0343-GK0344 and GK1380-GK1381) of Geobacillus kaustophilus HTA426.This strain have the DNA tranfer ability to Geobacillus kaustophilus HTA426.

Thermo tolerant lactic acid bacteriaLactic acid bacteria isolated from silage prepared in Okinawa