ligD and argB double disruptant of Aspergillus kawachii IFO4308, a host microorganism for efficient gene targeting

ligD and pyrG double disruptant of Aspergillus kawachii IFO4308, a host microorganism for efficient gene targeting

ligD, argB, and Sc triple disruptant of Aspergillus kawachii IFO 4308, a host microorganism for efficient gene targeting

nkuB disruptant of Aspergillus nidulans A89, a host microorganism for efficient gene targeting

wscA and wscB double disruptant of Aspergillus nidulans AKU89, cell wall mutant strain

Integrative shuttle vector in E. coli-Geobacillus. For gene insertion into Geobacillus kaustophilus HTA426 chromosome. This plasmid is consisted with pUC replicon, ampicilin resistance gene, uracil auxotrophic gene marker, gk704 promoter and GK0707 insertion region.

recombinant plasmid containing oriT into pUCG18 (Taylor, M. P., C. D. Esteban, and D. J. Leak. 2008, Development of a versatile shuttle vector for gene expression in Geobacillus spp. Plasmid 60: 45-52.), E. coli-Geobacillus shuttle vector. The plasmid consists of pUC replicon, ampicilin resistant gene, thermostable kanamycin nucleotidyltransferase gene (TK101), pBST1 replicon and oriT.

E.coli-Geobacillus shuttle plasmid derivered from pUC19. The plasmid consists of pUC replicon, thermostable kanamycin nucleotidyltransferase gene (TK101) and oriT.

Geobacillus kaustophilus HTA426 derivative lacking uracil biosynthesis genes (pyrF,pyrR) and restriction-modification system genes（GK1378-1390, GK0343-0346).